WB
Variouslysates were subjected to SDS PAGE followed by western blotwith NIBAN1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of(-20°C Ethanol) fixed HepG2 cells using NIBAN1 antibody at dilution of 1:200 and CoraLite®488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).IHC
Immunohistochemical analysis of paraffinembedded human pancreas cancertissue slide using NIBAN1 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).| Product Name | NIBAN1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FAM129A protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:20-1:200 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM129A |
|---|---|
| Gene Synonyms | Protein FAM129A, NIBAN1, NIBAN, GIG39, Cell growth-inhibiting gene 39 protein |
| Gene Full Name | Homo sapiens family with sequence similarity 129, member A |
| Gene Summary | his gene encodes a member of the family with sequence similarity 129 protein family. This gene is highly expressed in several cancer cells and may serve as a prognostic marker for certain cancers. The encoded protein may play a role in regulating p53-mediated apoptosis. [provided by RefSeq, Sep 2016] |
| Alternative Names | Protein FAM129A NIBAN1 NIBAN GIG39 Cell growth-inhibiting gene 39 protein |
| Molecular Weight(MW) | 103 kDa(Observed MW:150 kDa) |
| Cellular Localization | Cytoplasm；Membrane |
WB
Variouslysates were subjected to SDS PAGE followed by western blotwith NIBAN1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of(-20°C Ethanol) fixed HepG2 cells using NIBAN1 antibody at dilution of 1:200 and CoraLite®488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IHC
Immunohistochemical analysis of paraffinembedded human pancreas cancertissue slide using NIBAN1 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).| Application Notes | WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:20-1:200 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
