WB
Western blot analysis of extracts of various cell lines, using HSL (phos pho-S563) antibody at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treat ed with Isoproterenol (10 μM for 20 min) at 37℃.| Product Name | HSL (phospho-S563) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphorylated peptide around S563 of rat HSL (XP_006228456.1). |
| Modification | p-S563 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIPE |
|---|---|
| Gene Synonyms | HSL LHS REH AOMS4 FPLD6 |
| Gene Full Name | lipase E, hormone sensitive type |
| Gene Summary | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 82kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Lipid droplet,Membrane. |
WB
Western blot analysis of extracts of various cell lines, using HSL (phos pho-S563) antibody at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treat ed with Isoproterenol (10 μM for 20 min) at 37℃.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
