WB
Western blot analysis of Phospho-Phospholamban (S16) on different lysates with Rabbit anti-Phospho-Phospholamban (S16) antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lane 3: Mouse heart tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-Phospholamban (S16) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser16 of human phospholamban protein. |
| Modification | p-S16 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Pln |
|---|---|
| Gene Synonyms | Plb |
| Gene Full Name | phospholamban |
| Gene Summary | Enables ATPase inhibitor activity and protein homodimerization activity. Involved in several processes, including locomotor rhythm; negative regulation of calcium ion import into sarcoplasmic reticulum; and visual learning. Acts upstream of or within several processes, including muscle cell cellular homeostasis; regulation of calcium ion transport; and regulation of heart contraction. Located in sarcoplasmic reticulum membrane. Is expressed in several structures, including alimentary system; ear; heart; nervous system; and skeleton. Used to study centronuclear myopathy. Human ortholog(s) of this gene implicated in dilated cardiomyopathy 1P and hypertrophic cardiomyopathy 18. Orthologous to human PLN (phospholamban). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Cardiac phospholamban antibody CMD1P antibody CMH18 antibody PLB antibody Pln antibody PPLA_HUMAN antibody |
| Molecular Weight(MW) | 6 kDa(Observed band size: 6/12/24 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane, Mitochondrion membrane, Membrane. |
WB
Western blot analysis of Phospho-Phospholamban (S16) on different lysates with Rabbit anti-Phospho-Phospholamban (S16) antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lane 3: Mouse heart tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
