WB
Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Bronchus (Mouse) Lysate at 40 ug, Lane 4: Heart (Mouse) Lysate at 40 ug, Lane 5: Kidney (Rat) Lysate at 40 ug, Lane 6: Lung (Rat) Lysate at 40 ug, Lane 7: Bronchus (Rat) Lysate at 40 ug, Primary: Anti-NADPH oxidase 4 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.FC
Blank control:293T. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | NADPH oxidase 4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Nox-4 : 81-180/578 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 FC:1μg/Test |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NOX4 |
|---|---|
| Gene Synonyms | KOX KOX-1 RENOX |
| Gene Full Name | NADPH oxidase 4 |
| Gene Summary | This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] |
| Alternative Names | KOX KOX-1 RENOX NOX4_HUMAN NOX4 Kidney oxidase-1 (KOX-1) Kidney superoxide-producing NADPH oxidase Renal NAD(P)H-oxidase 1.6.3.1 NOX4_MOUSE Superoxide-generating NADPH oxidase 4 NOX4_RAT NADPH oxidase 4 |
| Molecular Weight(MW) | 64kDa |
WB
Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Bronchus (Mouse) Lysate at 40 ug, Lane 4: Heart (Mouse) Lysate at 40 ug, Lane 5: Kidney (Rat) Lysate at 40 ug, Lane 6: Lung (Rat) Lysate at 40 ug, Lane 7: Bronchus (Rat) Lysate at 40 ug, Primary: Anti-NADPH oxidase 4 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
FC
Blank control:293T. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 FC:1μg/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
