WB
Western blot analysis of CYR61 / CCN1 on different lysates with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution. Lane 1: Saos-2 cell lysate, Lane 2: 786-0 cell lysate, Lane 3: MCF7 cell lysate (negative), Lysates/proteins at 15 µg/Lane. Exposure time: 27 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CYR61 / CCN1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CYR61 / CCN1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of untreated Saos-2 (top, positive) / Saos-2 treated with 10μg/mL BFA overnight (middle, positive) / MCF7 (bottom, negative) labeling CYR61 / CCN1 with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Species: Human, Site: colon, Sample: Paraffin-embedded section, Antibody concentration: 1/50.| Product Name | CYR61 / CCN1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CCN1 aa 1-381. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:2000 IHC:1:200 ICC/IF:1:2000 IF-P:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCN1 |
|---|---|
| Gene Synonyms | GIG1 CYR61 IGFBP10 |
| Gene Full Name | cellular communication network factor 1 |
| Gene Summary | The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] |
| Alternative Names | 3CH61 antibody CCN 1 antibody CCN family member 1 antibody CCN1 antibody CYR 61 antibody Cyr61 antibody CYR61 protein antibody CYR61_HUMAN antibody Cysteine rich angiogenic inducer 61 antibody Cysteine rich heparin binding protein 61 antibody 3CH61 antibody CCN 1 antibody CCN family member 1 antibody CCN1 antibody CYR 61 antibody Cyr61 antibody CYR61 protein antibody CYR61_HUMAN antibody Cysteine rich angiogenic inducer 61 antibody Cysteine rich heparin binding protein 61 antibody Cysteine-rich angiogenic inducer 61 antibody GIG 1 antibody GIG1 antibody IBP-10 antibody IGF-binding protein 10 antibody Igfbp 10 antibody IGFBP-10 antibody Igfbp10 antibody Insulin like growth factor binding protein 10 antibody Insulin-like growth factor-binding protein 10 antibody Protein CYR61 antibody Protein GIG1 antibody |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CYR61 / CCN1 on different lysates with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution. Lane 1: Saos-2 cell lysate, Lane 2: 786-0 cell lysate, Lane 3: MCF7 cell lysate (negative), Lysates/proteins at 15 µg/Lane. Exposure time: 27 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CYR61 / CCN1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CYR61 / CCN1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of untreated Saos-2 (top, positive) / Saos-2 treated with 10μg/mL BFA overnight (middle, positive) / MCF7 (bottom, negative) labeling CYR61 / CCN1 with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYR61 / CCN1 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Species: Human, Site: colon, Sample: Paraffin-embedded section, Antibody concentration: 1/50.| Application Notes | WB:1:2000 IHC:1:200 ICC/IF:1:2000 IF-P:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
