WB
Various lysates were subjected to SDS PAGE followed by western blot with VPS26B antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VPS26B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS26B antibody (at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).| Product Name | VPS26B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human VPS26B protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.45mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VPS26B |
|---|---|
| Gene Synonyms | Pep8b |
| Gene Full Name | VPS26 retromer complex component B |
| Gene Summary | Predicted to be involved in intracellular protein transport and retrograde transport, endosome to Golgi. Predicted to act upstream of or within cellular response to type II interferon. Predicted to be located in early endosome and late endosome. Predicted to be part of retromer complex. Predicted to be active in glutamatergic synapse; postsynaptic early endosome; and postsynaptic recycling endosome. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Vacuolar protein sorting-associated protein 26B Vesicle protein sorting 26B |
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Cytoplasm,Endosome,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with VPS26B antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using VPS26B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS26B antibody (at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
