WB
Western blot analysis of Phospho-MEK3 (S189)+MEK6 (S207) on different lysates with Rabbit anti-Phospho-MEK3 (S189)+MEK6 (S207) antibody at 1/15,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-MEK3 (S189)+MEK6 (S207) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser189 of Human MEK3 (P46734-2). |
| Modification | p-S189+S207 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:15000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP2K3/MAP2K6 |
|---|---|
| Gene Synonyms | MEK6 MKK6 CRCMSL MAPKK6 PRKMK6 SAPKK3 SAPKK-3 MEK3 MKK3 MAPKK3 PRKMK3 SAPKK2 SAPKK-2 |
| Gene Full Name | mitogen-activated protein kinase kinase 6 |
| Gene Summary | This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 39/37 kDa |
| Cellular Localization | Cytoplasm, cytosol, membrane, nucleoplasm; Nucleus, Cytoplasm, cytoskeleton. |
WB
Western blot analysis of Phospho-MEK3 (S189)+MEK6 (S207) on different lysates with Rabbit anti-Phospho-MEK3 (S189)+MEK6 (S207) antibody at 1/15,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:15000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
