WB
Various lysates were subjected to SDS PAGE followed by western blot with Angiopoietin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Angiopoietin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Angiopoietin 1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).FC
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Angiopoietin 1 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ANGPT1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 IF-P:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANGPT1 |
|---|---|
| Gene Synonyms | AGP1 AGPT ANG1 HAE5 AGPT-1 |
| Gene Full Name | angiopoietin 1 |
| Gene Summary | This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Mutations in this gene are associated with hereditary angioedema. [provided by RefSeq, Aug 2020] |
| Alternative Names | ANGPT1 240764D10 AGP1 AGPT ANG 1 |
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Angiopoietin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Angiopoietin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Angiopoietin 1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 IF-P:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
