WB
Various lysates were subjected to SDS PAGE followed by western blot with SRF antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using SRF antibody at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SRF antibody at dilution of 1:600 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).IP
IP result of anti-SRF (IP:3ug; Detection: 1:1000) with HeLa cells lysate 3000ug.| Product Name | SRF Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SRF protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:6000 IHC:1:2000-1:8000 ICC/IF:1:300-1:1200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.6mg/ml |
| Purification | Protein A |
| Gene Symbol | SRF |
|---|---|
| Gene Synonyms | MCM1 |
| Gene Full Name | serum response factor |
| Gene Summary | This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Alternative Names | Serum response factor MCM1 |
| Molecular Weight(MW) | 65-70 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SRF antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using SRF antibody at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SRF antibody at dilution of 1:600 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).
IP
IP result of anti-SRF (IP:3ug; Detection: 1:1000) with HeLa cells lysate 3000ug.| Application Notes | WB:1:1000-1:6000 IHC:1:2000-1:8000 ICC/IF:1:300-1:1200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
