WB
Western blot analysis of various lysates using IL-6 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. RAW 264.7 cells and NR8383 cells were treated with LPS (1μg/mL) and BFA (300ng/mL) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.ICC/IF
Confocal imaging of RAW 264.7 cells (treated with LPS and BFA) and RAW 264.7 cells (untreated) using IL-6 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.IP
Immunoprecipitation of IL-6 from 1000 µg extracts of NR8383 cells treated with LPS (100 ng/ml, 24 h) and BFA (1 µg/ml, 6 h) was performed using 1 µg of IL-6 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using IL-6 Rabbit mAb at a dilution of 1:10000.| Product Name | IL-6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:1000-1:5000 ICC/IF:1:200-1:800 IP:0.5 μg-4 μg antibody for 900 μg-1100 μg extracts of whole cells |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Il6 |
|---|---|
| Gene Synonyms | ILg6 Ifnb2 |
| Gene Full Name | interleukin 6 |
| Gene Summary | Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of cell communication; and positive regulation of metabolic process. Located in extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; hypertension (multiple); impotence; and steatotic liver disease. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | ILg6 Ifnb2 |
| Molecular Weight(MW) | 24kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates using IL-6 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. RAW 264.7 cells and NR8383 cells were treated with LPS (1μg/mL) and BFA (300ng/mL) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
ICC/IF
Confocal imaging of RAW 264.7 cells (treated with LPS and BFA) and RAW 264.7 cells (untreated) using IL-6 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
IP
Immunoprecipitation of IL-6 from 1000 µg extracts of NR8383 cells treated with LPS (100 ng/ml, 24 h) and BFA (1 µg/ml, 6 h) was performed using 1 µg of IL-6 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using IL-6 Rabbit mAb at a dilution of 1:10000.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:200-1:800 IP:0.5 μg-4 μg antibody for 900 μg-1100 μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
