WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-8 (Asp387)antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 was treated with LPS(100mg/ml) for 24 hours.| Product Name | Cleaved Caspase-8 (Asp387) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Ser79 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CASP8 |
|---|---|
| Gene Synonyms | CAP4 MACH MCH5 FLICE ALPS2B Casp-8 |
| Gene Full Name | caspase 8 |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | CASP8 MCH5 Caspase-8 CASP-8 Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 FADD-homologous ICE/ced-3-like protease FADD-like ICE FLICE ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog MACH |
| Molecular Weight(MW) | 43kDa,18kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-8 (Asp387)antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 was treated with LPS(100mg/ml) for 24 hours.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
