WB
Western blot analysis of UBA2 using anti-UBA2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: Skov-3 whole cell lysates, Lane 5: Colo320 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat river tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse river tissue lysates, Lane 13: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBA2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
UBA2 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
UBA2 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
UBA2 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- SAE2/UBA2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.FC
Flow Cytometry analysis of A431 cells using anti-SAE2/UBA2 antibody. Overlay histogram showing A431 cells stained with anti-SAE2/UBA2 antibody (Blue line). And then incubated with rabbit anti-SAE2/UBA2 Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to UBA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBA2 |
|---|---|
| Gene Synonyms | ARX SAE2 ACCES HRIHFB2115 |
| Gene Full Name | ubiquitin like modifier activating enzyme 2 |
| Gene Summary | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
| Alternative Names | ARX HRIHFB2115 SAE2 UBA2 UBLE1B |
| Molecular Weight(MW) | 71 kDa(Observed band size: 90 kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of UBA2 using anti-UBA2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Hela whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: Skov-3 whole cell lysates, Lane 5: Colo320 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat river tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse river tissue lysates, Lane 13: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBA2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
UBA2 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
UBA2 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
UBA2 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UBA2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- SAE2/UBA2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FC
Flow Cytometry analysis of A431 cells using anti-SAE2/UBA2 antibody. Overlay histogram showing A431 cells stained with anti-SAE2/UBA2 antibody (Blue line). And then incubated with rabbit anti-SAE2/UBA2 Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
