WB
Western blot analysis of anti-PAIP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAIP1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
PAIP1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kitwith DAB as the chromogen.IHC
PAIP1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
PAIP1 was detected in an immunocytochemical section of Hela cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing SH-SY5Y cells stained withanti-PAIP1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAIP1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to PAIP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PAIP1 recombinant protein (Position: R92-Q420). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAIP1 |
|---|---|
| Gene Full Name | poly(A) binding protein interacting protein 1 |
| Gene Summary | The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | PAIP1 Polyadenylate-binding protein-interacting protein 1 PABP-interacting protein 1 PAIP-1 Poly(A)-binding protein-interacting protein 1 |
| Molecular Weight(MW) | 51 kDa(Observed MW 45 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of anti-PAIP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAIP1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
PAIP1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kitwith DAB as the chromogen.
IHC
PAIP1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
PAIP1 was detected in an immunocytochemical section of Hela cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing SH-SY5Y cells stained withanti-PAIP1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAIP1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
