WB
Various lysates were subjected to SDS PAGE followed by western blot with NR5A1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using NR5A1 Antibody at dilution of 1:100 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using NR5A1 Antibody at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-NR5A1 (IP:4ug; Detection: 1:300) with A2780 cells lysate 960ug.FC
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | NR5A1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NR5A1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:500-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Symbol | NR5A1 |
|---|---|
| Gene Synonyms | ELP SF1 FTZ1 POF7 SF-1 AD4BP FTZF1 SPGF8 SRXX4 SRXY3 hSF-1 |
| Gene Full Name | nuclear receptor subfamily 5 group A member 1 |
| Gene Summary | The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008] |
| Alternative Names | AD4BP Adrenal 4 binding protein Adrenal 4-binding protein ELP FTZ1 |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NR5A1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using NR5A1 Antibody at dilution of 1:100 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using NR5A1 Antibody at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-NR5A1 (IP:4ug; Detection: 1:300) with A2780 cells lysate 960ug.
FC
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
