WB
Western blot analysis of various lysates using SP1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of C6 cells using SP1 Rabbit mAb (at dilution of 1:100) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.ChIP
Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.CUT&Tag
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of SP1 Rabbit mAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).| Product Name | SP1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCWBCUT&Tag-seq |
| WB:1:1000-1:2000 IHC:1:200-1:2000 ICC/IF:1:200-1:1000 ChIP:5μg antibody for 10μg-15μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SP1 |
|---|---|
| Gene Full Name | Sp1 transcription factor |
| Gene Summary | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| Alternative Names | SP1 |
| Molecular Weight(MW) | 81kDa(Observed MW 90kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using SP1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of C6 cells using SP1 Rabbit mAb (at dilution of 1:100) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ChIP
Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of SP1 Rabbit mAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:2000 ICC/IF:1:200-1:1000 ChIP:5μg antibody for 10μg-15μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
