WB
Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, All lanes: NUP85 antibody at 2.7μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human colon cancer using NUP85 Antibody at dilution of 1:100.IHC
Immunohistochemistry of paraffin-embedded human pancreatic tissue using NUP85 Antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of MCF-7 cells using NUP85 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-NUP85 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Nuclear pore complex protein Nup85 protein (201-500AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP85 |
|---|---|
| Gene Synonyms | Nup75 FROUNT NPHS17 |
| Gene Full Name | nucleoporin 85 |
| Gene Summary | This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | 85 kDa nucleoporin antibody FLJ12549 antibody FROUNT antibody Frount protein antibody Nuclear pore complex protein nucleoporin 85 kDa antibody nuclear pore complex protein nucleoporin Nup75 antibody Nuclear pore complex protein Nup85 antibody Nucleoporin 85 antibody Nucleoporin 85kDa antibody Nucleoporin nucleoporin 85kDa antibody Nucleoporin Nup75 antibody Nucleoporin NUP85 antibody nucleoporin 75-KD antibody Nucleoporin85 antibody Nup 75 antibody NUP 85 antibody Nup75 antibody nup85 antibody NUP85_HUMAN antibody PCN antibody PCNT 1 antibody PCNT antibody PCNT formerly antibody PCNT1 antibody Pericentrin antibody Pericentrin formerly antibody Pericentrin-1 antibody Pericentrin1 antibody |
| Molecular Weight(MW) | 70/76 kDa |
| Cellular Localization | Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Nucleus membrane. |
WB
Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, All lanes: NUP85 antibody at 2.7μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human colon cancer using NUP85 Antibody at dilution of 1:100.
IHC
Immunohistochemistry of paraffin-embedded human pancreatic tissue using NUP85 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of MCF-7 cells using NUP85 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:2000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
