WB
Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody.IHC
Immunohistochemistry analysis of paraffin embedded Rat testis using TriMethyl-Histone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using TriMethyl Histone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb. Blue: DAPI for nuclear staining.| Product Name | TriMethyl-Histone H4-K20 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Modification | TriMethyl-K20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFIHCWB |
| WB:1:500-1:2000 DB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4FA H4-16 H4C11 H4C12 H4C13 H4C14 H4C15 H4C16 HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Alternative Names | H4 H4/n H4C1 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4F2 H4FN FO108 H4-16 H4C11 H4C12 H4C13 H4C15 H4C16 HIST2H4 HIST2H4A TriMethyl-Histone H4-K20 |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody.
IHC
Immunohistochemistry analysis of paraffin embedded Rat testis using TriMethyl-Histone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using TriMethyl Histone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 DB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
