WB
Western blot analysis of ANXA6 on different lysates with Rabbit anti-ANXA6 antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: Raji cell lysate (20 µg/Lane), Lane 3: THP-1 cell lysate (20 µg/Lane), Lane 4: PC-3M cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: HEK-293 cell lysate (20 µg/Lane), Lane 7: HeLa cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: L6 cell lysate (20 µg/Lane), Lane 10: Mouse pancreas tissue lysate (40 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Rat heart tissue lysate (40 µg/Lane), Lane 13: Rat liver tissue lysate (40 µg/Lane), Exposure time: Lane 1-13: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ANXA6 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-ANXA6 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ANXA6 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
ANXA6 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-ANXA6 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-ANXA6 antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-ANXA6 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-ANXA6 antibody in Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 14 seconds.| Product Name | ANXA6 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human ANXA6 aa 1-673. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 IHC:1:2000-1:10000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANXA6 |
|---|---|
| Gene Synonyms | p68 p70 ANX6 CBP68 CPB-II |
| Gene Full Name | annexin A6 |
| Gene Summary | Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010] |
| Alternative Names | 67 kDa calelectrin antibody Annexin A6 antibody Annexin VI antibody Annexin VI p68 antibody Annexin-6 antibody AnnexinA6 antibody AnnexinVI antibody ANX 6 antibody ANX A6 antibody ANX6 antibody 67 kDa calelectrin antibody Annexin A6 antibody Annexin VI antibody Annexin VI p68 antibody Annexin-6 antibody AnnexinA6 antibody AnnexinVI antibody ANX 6 antibody ANX A6 antibody ANX6 antibody ANXA 6 antibody ANXA6 antibody ANXA6_HUMAN antibody Calcium binding protein p68 antibody Calelectrin antibody Calphobindin II antibody Calphobindin-II antibody CalphobindinII antibody CBP 68 antibody CBP68 antibody Chromobindin 20 antibody Chromobindin-20 antibody Chromobindin20 antibody CPB II antibody CPB-II antibody CPBII antibody Lipocortin VI antibody LipocortinVI antibody p68 antibody p70 antibody Protein III antibody ProteinIII antibody |
| Molecular Weight(MW) | 76 kDa(Observed band size: 70 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ANXA6 on different lysates with Rabbit anti-ANXA6 antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane), Lane 2: Raji cell lysate (20 µg/Lane), Lane 3: THP-1 cell lysate (20 µg/Lane), Lane 4: PC-3M cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: HEK-293 cell lysate (20 µg/Lane), Lane 7: HeLa cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: L6 cell lysate (20 µg/Lane), Lane 10: Mouse pancreas tissue lysate (40 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Rat heart tissue lysate (40 µg/Lane), Lane 13: Rat liver tissue lysate (40 µg/Lane), Exposure time: Lane 1-13: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ANXA6 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-ANXA6 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ANXA6 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
ANXA6 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-ANXA6 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-ANXA6 antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-ANXA6 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-ANXA6 antibody in Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 14 seconds.| Application Notes | WB:1:2000 IHC:1:2000-1:10000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
