WB
Western blot analysis of CXCL9 on different lysates with Rabbit anti-CXCL9 antibody at 1/1,000 dilution. Lane 1: THP-1 cell lysate, Lane 2: THP-1 treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of THP-1 cells untreated / treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours labeling CXCL9 with Rabbit anti-CXCL9 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL9 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | CXCL9 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CXCL9 aa 23-125. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCL9 |
|---|---|
| Gene Synonyms | CMK MIG Humig SCYB9 crg-10 |
| Gene Full Name | C-X-C motif chemokine ligand 9 |
| Gene Summary | This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Aug 2020] |
| Alternative Names | C-X-C motif chemokine 9 antibody chemokine (C-X-C motif) ligand 9 antibody CMK antibody crg-10 antibody CXCL9 antibody CXCL9_HUMAN antibody gamma interferon induced monokine antibody Gamma-interferon-induced monokine antibody HuMIG antibody MIG antibody C-X-C motif chemokine 9 antibody chemokine (C-X-C motif) ligand 9 antibody CMK antibody crg-10 antibody CXCL9 antibody CXCL9_HUMAN antibody gamma interferon induced monokine antibody Gamma-interferon-induced monokine antibody HuMIG antibody MIG antibody monokine induced by gamma interferon antibody monokine induced by interferon gamma antibody Monokine induced by interferon-gamma antibody SCYB9 antibody Small inducible cytokine B9 antibody small inducible cytokine subfamily B member 9 antibody Small-inducible cytokine B9 antibody |
| Molecular Weight(MW) | 14 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CXCL9 on different lysates with Rabbit anti-CXCL9 antibody at 1/1,000 dilution. Lane 1: THP-1 cell lysate, Lane 2: THP-1 treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of THP-1 cells untreated / treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours labeling CXCL9 with Rabbit anti-CXCL9 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL9 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
