WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2+Arg8+Arg17) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7, Lane 2: SH-SY5Y ,Lane 3: NIH-3T3, Lane 4: RAW264.7, Lane 5: C6, Lane 6: PC-12 .IHC
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2+Arg8+Arg17) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 3 hours.| Product Name | Histone H3 (Citrulline Arg2+Arg8+Arg17) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Citrulline Arg2+Arg8+Arg17 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Histone H3.1 |
|---|---|
| Gene Synonyms | H3C1 H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3C10 H3C11 H3C12 |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Nucleus. Chromosome. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2+Arg8+Arg17) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7, Lane 2: SH-SY5Y ,Lane 3: NIH-3T3, Lane 4: RAW264.7, Lane 5: C6, Lane 6: PC-12 .
IHC
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2+Arg8+Arg17) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 3 hours.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
