WB
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody at 1/5,000 dilution. Lane 1: COLO205 cell lysate, Lane 2: K-562 cell lysate (negative), Lane 3: Saos-2 cell lysate, Lane 4: SW480 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 20 seconds; Lane 4: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of COLO205 (positive) and K-562 (negative) labeling Eph receptor B2 with Rabbit anti-Eph receptor B2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor B2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
Eph receptor B2 was immunoprecipitated from 0.2 mg COLO205 cell lysate with Rabbit anti-Eph receptor B2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Eph receptor B2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: COLO205 cell lysate (input), Lane 2: Rabbit anti-Eph receptor B2 antibody IP in COLO205 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Eph receptor B2 antibody in COLO205 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Product Name | Eph receptor B2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Eph receptor B2 aa 1-543. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:5000 ICC/IF:1:100 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EPHB2 |
|---|---|
| Gene Synonyms | DRT EK5 ERK CAPB Hek5 PCBC EPHT3 Tyro5 BDPLT22 |
| Gene Full Name | EPH receptor B2 |
| Gene Summary | This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015] |
| Alternative Names | cek5 antibody Developmentally regulated EPH related tyrosine kinase antibody DRT antibody EK5 antibody ELK related protein tyrosine kinase antibody Eph receptor B2 antibody EPH tyrosine kinase 3 antibody EPH-like kinase 5 antibody EPHB2 antibody EPHB2_HUMAN antibody cek5 antibody Developmentally regulated EPH related tyrosine kinase antibody DRT antibody EK5 antibody ELK related protein tyrosine kinase antibody Eph receptor B2 antibody EPH tyrosine kinase 3 antibody EPH-like kinase 5 antibody EPHB2 antibody EPHB2_HUMAN antibody Ephrin type B receptor 2 antibody Ephrin type-B receptor 2 antibody EPHT 3 antibody ERK antibody ETECK antibody hEK5 antibody Nuk antibody Prkm 5 antibody Receptor protein tyrosine kinase HEK 5 antibody Renal carcinoma antigen NY-REN-47 antibody Sek 3 antibody Tyro 5 antibody Tyrosine protein kinase receptor CEK 5 antibody Tyrosine protein kinase receptor EPH 3 antibody Tyrosine protein kinase receptor QEK 5 antibody Tyrosine-protein kinase receptor EPH-3 antibody Tyrosine-protein kinase TYRO5 antibody |
| Molecular Weight(MW) | 117 kDa(Observed band size: 130 kDa) |
| Cellular Localization | Cell membrane, Cell projection, axon, dendrite. |
WB
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody at 1/5,000 dilution. Lane 1: COLO205 cell lysate, Lane 2: K-562 cell lysate (negative), Lane 3: Saos-2 cell lysate, Lane 4: SW480 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 20 seconds; Lane 4: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of COLO205 (positive) and K-562 (negative) labeling Eph receptor B2 with Rabbit anti-Eph receptor B2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor B2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
Eph receptor B2 was immunoprecipitated from 0.2 mg COLO205 cell lysate with Rabbit anti-Eph receptor B2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Eph receptor B2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: COLO205 cell lysate (input), Lane 2: Rabbit anti-Eph receptor B2 antibody IP in COLO205 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Eph receptor B2 antibody in COLO205 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Application Notes | WB:1:5000 ICC/IF:1:100 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
