WB
Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: HepG2 cell lysate (15 µg/Lane), Lane 4: HEK-293 cell lysate (15 µg/Lane), Lane 5: 293T cell lysate (15 µg/Lane), Lane 6: MCF7 cell lysate (15 µg/Lane), Lane 7: NIH/3T3 cell lysate (15 µg/Lane), Lane 8: Mouse testis tissue lysate (30 µg/Lane), Lane 9: Rat testis tissue lysate (30 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with Rabbit anti-PRMT7 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PRMT7 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse testis tissue lysate (input), Lane 2: Rabbit anti-PRMT7 antibody IP in mouse testis tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PRMT7 antibody in mouse testis tissue lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 11 seconds.| Product Name | PRMT7 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human PRMT7 aa 1-50 / 692. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000 IHC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRMT7 |
|---|---|
| Gene Synonyms | SBIDDS |
| Gene Full Name | protein arginine methyltransferase 7 |
| Gene Summary | This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017] |
| Alternative Names | [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody ANM7_HUMAN antibody FLJ10640 antibody Histone-arginine N-methyltransferase PRMT7 antibody KIAA1933 antibody Myelin basic protein arginine N methyltransferase antibody OTTHUMP00000174863 antibody PRMT7 antibody Protein arginine methyltransferase 7 antibody Protein arginine N-methyltransferase 7 antibody |
| Molecular Weight(MW) | 78 kDa |
| Cellular Localization | Cytoplasm, cytosol, Nucleus. |
WB
Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: HepG2 cell lysate (15 µg/Lane), Lane 4: HEK-293 cell lysate (15 µg/Lane), Lane 5: 293T cell lysate (15 µg/Lane), Lane 6: MCF7 cell lysate (15 µg/Lane), Lane 7: NIH/3T3 cell lysate (15 µg/Lane), Lane 8: Mouse testis tissue lysate (30 µg/Lane), Lane 9: Rat testis tissue lysate (30 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PRMT7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
PRMT7 was immunoprecipitated from 0.2 mg mouse testis tissue lysate with Rabbit anti-PRMT7 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PRMT7 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse testis tissue lysate (input), Lane 2: Rabbit anti-PRMT7 antibody IP in mouse testis tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PRMT7 antibody in mouse testis tissue lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 11 seconds.| Application Notes | WB:1:1000 IHC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
