WB
Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody diluted at 1:1000.IHC
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Product Name | Cleaved Caspase-1 p20 (Asp210) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range:161-210. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CASP1 |
|---|---|
| Gene Synonyms | ICE P45 IL1BC |
| Gene Full Name | caspase 1 |
| Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| Alternative Names | CASP1 IL1BC IL1BCE Caspase-1 CASP-1 Interleukin-1 beta convertase IL-1BC Interleukin-1 beta-converting enzyme ICE IL-1 beta-converting enzyme p45 [Cleaved into: Caspase-1 subunit p20 Caspase-1 subunit p10] |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Cytoplasm, Cell membrane. |
WB
Western Blot analysis of NIH-3T3 cells using Cleaved-Caspase-1 (D210) Polyclonal Antibody diluted at 1:1000.
IHC
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
