WB
Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time:30s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IF-P
Immunofluorescence analysis of paraffinembedded mouse heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | N-Cadherin Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIF-PIHCWB |
| WB:1:500-1:1000 IHC1:100-1:500IF-P:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH2 |
|---|---|
| Gene Synonyms | CDHN NCAD ACOGS ADHD8 CD325 ARVD14 CDw325 |
| Gene Full Name | cadherin 2 |
| Gene Summary | This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015] |
| Molecular Weight(MW) | 100kDa（80kDa/135kDa/130kDa） |
| Cellular Localization | Cell membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time:30s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffinembedded mouse heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Positive Control | HeLa, A-549, 293T, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat heart, Mouse heart |
|---|---|
| Application Notes | WB:1:500-1:1000 IHC1:100-1:500IF-P:1:100-1:500 ELISA:1:1000-1:10000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
