WB
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate, Lane 2: A375 cell lysate, Lane 3: A549 cell lysate, Lane 4: HeLa cell lysate, Lane 5: SH-SY5Y cell lysate, Lane 6: NIH/3T3 cell lysate, Lane 7: MCF7 cell lysate, Lane 8: Mouse brain tissue lysate, Lane 9: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRBN antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
CRBN was immunoprecipitated in 0.2mg HeLa cell lysate with Rabbit anti-CRBN antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CRBN antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2:Rabbit anti-CRBN antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CRBN antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute 10 seconds.| Product Name | CRBN Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CRBN aa 1-442 / 442. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000 IHC:1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CRBN |
|---|---|
| Gene Synonyms | MRT2 MRT2A |
| Gene Full Name | cereblon |
| Gene Summary | This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| Alternative Names | 2610203G15Rik antibody 2900045O07Rik antibody AF229032 antibody AW108261 antibody Cereblon antibody Crbn antibody CRBN_HUMAN antibody DKFZp781K0715 antibody MGC27358 antibody MRT2A antibody 2610203G15Rik antibody 2900045O07Rik antibody AF229032 antibody AW108261 antibody Cereblon antibody Crbn antibody CRBN_HUMAN antibody DKFZp781K0715 antibody MGC27358 antibody MRT2A antibody OTTHUMP00000209555 antibody piL antibody Protein cereblon antibody Protein x 0001 antibody |
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane. |
WB
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate, Lane 2: A375 cell lysate, Lane 3: A549 cell lysate, Lane 4: HeLa cell lysate, Lane 5: SH-SY5Y cell lysate, Lane 6: NIH/3T3 cell lysate, Lane 7: MCF7 cell lysate, Lane 8: Mouse brain tissue lysate, Lane 9: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRBN antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
CRBN was immunoprecipitated in 0.2mg HeLa cell lysate with Rabbit anti-CRBN antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CRBN antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2:Rabbit anti-CRBN antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CRBN antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute 10 seconds.| Application Notes | WB:1:1000 IHC:1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
