WB
Western blot analysis of Prosurfactant Protein C on different lysates with Rabbit anti-Prosurfactant Protein C antibody at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse spleen tissue lysate (negative), Lane 3: Rat lung tissue lysate, Lane 4: Rat spleen tissue lysate (negative), Lysates/proteins at 40 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells transfected with or without Prosurfactant Protein C labeling Prosurfactant Protein C with Rabbit anti-Prosurfactant Protein C antibody at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Prosurfactant Protein C antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Species: Mouse, Site: lung, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.FC
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with Prosurfactant Protein C (right) labeling Prosurfactant Protein C. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Prosurfactant Protein C Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Prosurfactant Protein C aa 1-50. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:5000 IHC:1:3000 ICC/IF:1:2000 IF-P:1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SFTPC |
|---|---|
| Gene Synonyms | SP5 SP-C PSP-C SFTP2 SMDP2 BRICD6 |
| Gene Full Name | surfactant protein C |
| Gene Summary | This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010] |
| Alternative Names | BRICD6 antibody BRICHOS domain containing 6 antibody PSP C antibody PSPC antibody PSPC_HUMAN antibody Pulmonary surfactant apoprotein 2 antibody Pulmonary surfactant apoprotein PSP C antibody pulmonary surfactant apoprotein-2 SP-C antibody Pulmonary surfactant associated protein C antibody Pulmonary surfactant associated proteolipid SPL pVal antibody BRICD6 antibody BRICHOS domain containing 6 antibody PSP C antibody PSPC antibody PSPC_HUMAN antibody Pulmonary surfactant apoprotein 2 antibody Pulmonary surfactant apoprotein PSP C antibody pulmonary surfactant apoprotein-2 SP-C antibody Pulmonary surfactant associated protein C antibody Pulmonary surfactant associated proteolipid SPL pVal antibody Pulmonary surfactant associated proteolipid SPL(Val) antibody Pulmonary surfactant protein SP5 antibody Pulmonary surfactant-associated protein C antibody Pulmonary surfactant-associated proteolipid SPL(Val) antibody SFTP 2 antibody SFTP2 antibody SFTPC antibody SFTPC surfactant pulmonary associated protein C antibody SMDP2 antibody SP 5 antibody SP C antibody SP-C antibody SP5 antibody SPC antibody Surfactant associated protein pulmonary 2 antibody Surfactant protein c antibody Surfactant proteolipid SPL-pVal antibody Surfactant pulmonary associated protein C antibody |
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Secreted, extracellular space, surface film. |
WB
Western blot analysis of Prosurfactant Protein C on different lysates with Rabbit anti-Prosurfactant Protein C antibody at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse spleen tissue lysate (negative), Lane 3: Rat lung tissue lysate, Lane 4: Rat spleen tissue lysate (negative), Lysates/proteins at 40 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Prosurfactant Protein C antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells transfected with or without Prosurfactant Protein C labeling Prosurfactant Protein C with Rabbit anti-Prosurfactant Protein C antibody at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Prosurfactant Protein C antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Species: Mouse, Site: lung, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.
FC
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with Prosurfactant Protein C (right) labeling Prosurfactant Protein C. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 IHC:1:3000 ICC/IF:1:2000 IF-P:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
