WB
Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane), Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane), Lane 3: HUVEC cell lysate (20 µg/Lane), Lane 4: C2C12 cell lysate (20 µg/Lane), Lane 5: Mouse spleen tissue lysate (40 µg/Lane), Lane 6: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Site: spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Product Name | Integrin beta 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Integrin beta 3 aa 101-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IHC:1:2000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ITGB3 |
|---|---|
| Gene Synonyms | GT GT2 CD61 GP3A BDPLT2 FMAIT1 GPIIIa BDPLT16 BDPLT24 |
| Gene Full Name | integrin subunit beta 3 |
| Gene Summary | The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| Alternative Names | BDPLT16 antibody BDPLT2 antibody CD 61 antibody CD61 antibody CD61 antigen antibody GP3A antibody GPIIIa antibody GT antibody HPA 1 antibody HPA 4 antibody BDPLT16 antibody BDPLT2 antibody CD 61 antibody CD61 antibody CD61 antigen antibody GP3A antibody GPIIIa antibody GT antibody HPA 1 antibody HPA 4 antibody Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody Integrin beta chain beta 3 antibody Integrin beta-3 antibody ITB3_HUMAN antibody ITG B3 antibody ITGB 3 antibody ITGB3 antibody NAIT antibody platelet fibrinogen receptor antibody Platelet fibrinogen receptor beta subunit antibody Platelet fibrinogen receptor beta subunit antibody Platelet glycoprotein IIIa antibody Platelet glycoprotein IIIa precursor antibody Platelet membrane glycoprotein IIIa antibody PTP antibody |
| Molecular Weight(MW) | 87 kDa(Observed band size: 100 kDa) |
| Cellular Localization | Cell membrane, Cell projection, lamellipodium membrane, Cell junction, focal adhesion, Postsynaptic cell membrane, Synapse. |
WB
Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane), Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane), Lane 3: HUVEC cell lysate (20 µg/Lane), Lane 4: C2C12 cell lysate (20 µg/Lane), Lane 5: Mouse spleen tissue lysate (40 µg/Lane), Lane 6: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Integrin beta 3 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Site: spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Application Notes | WB:1:5000 IHC:1:2000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
