WB
Western blot analysis of various lysates using GATAD2B Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse pancreas using GATAD2B Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3μg GATAD2B antibody. Western blot was performed from the immunoprecipitate using GATAD2B antibody at a dilution of 1:1000.| Product Name | GATAD2B Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATAD2B |
|---|---|
| Gene Synonyms | p68 GANDS MRD18 P66beta |
| Gene Full Name | GATA zinc finger domain containing 2B |
| Gene Summary | This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with cognitive disability. [provided by RefSeq, Jun 2016] |
| Alternative Names | p68 GANDS MRD18 P66beta GATAD2B |
| Molecular Weight(MW) | 65kDa(Observed MW 80kDa) |
| Cellular Localization | Nucleus speckle. |
WB
Western blot analysis of various lysates using GATAD2B Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse pancreas using GATAD2B Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3μg GATAD2B antibody. Western blot was performed from the immunoprecipitate using GATAD2B antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
