WB
Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-PPAR alpha (Ser12) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Product Name | phospho-PPAR alpha (Ser12) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12 : PL(p-S)PL |
| Modification | p-Ser12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPARA |
|---|---|
| Gene Synonyms | PPAR NR1C1 hPPAR PPARalpha PPAR-alpha |
| Gene Full Name | peroxisome proliferator activated receptor alpha |
| Gene Summary | Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | NR1C1 PPAR PPAR-alpha PPARalpha hPPAR 4933429D07Rik PPARA_HUMAN PPARA Nuclear receptor subfamily 1 group C member 1 Q6I9S0_HUMAN PPARA_MOUSE Q542P9_MOUSE PPARA_RAT peroxisome proliferator activated receptor alpha peroxisome proliferative activated receptor alpha peroxisome proliferator-activated receptor alpha PPARA | PPAR alpha (phospho-S12) p-PPAR alpha phospho-PPAR alpha p-PPARA PPARA | PPAR alpha (phospho-Ser12) |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Nucleus. |
WB
Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-PPAR alpha (Ser12) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
