WB
Western blot analysis of ANLN using anti-ANLN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANLN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibod. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of ANLN using anti-ANLN antibody and anti-Beta Tubulin antibody. ANLN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ANLN Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IP
IP analysis of ANLN using anti-ANLN antibody in U251 whole cell lysate. Western blot analysis of ANLN using anti- ANLN antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- ANLN antibody in U251 whole cell lysate, Lane 3: anti- ANLN antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ANLN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of U251 cells using anti-ANLN antibody . Overlay histogram showing U251 cells stained with anti-ANLN antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANLN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ANLN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Anillin/ANLN, which shares 96.4% amino acid (aa) sequence identity with both mouse and rat Anillin/ANLN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANLN |
|---|---|
| Gene Synonyms | scra FSGS8 Scraps |
| Gene Full Name | anillin, actin binding protein |
| Gene Summary | This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] |
| Alternative Names | Actin binding protein anillin ANLN scra Scraps |
| Molecular Weight(MW) | 124 kDa(Observed MW 150 kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Nucleus. |
WB
Western blot analysis of ANLN using anti-ANLN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANLN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibod. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of ANLN using anti-ANLN antibody and anti-Beta Tubulin antibody. ANLN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ANLN Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IP
IP analysis of ANLN using anti-ANLN antibody in U251 whole cell lysate. Western blot analysis of ANLN using anti- ANLN antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- ANLN antibody in U251 whole cell lysate, Lane 3: anti- ANLN antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ANLN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of U251 cells using anti-ANLN antibody . Overlay histogram showing U251 cells stained with anti-ANLN antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANLN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
