WB
Western blot analysis of various lysates using K11-linkage Specific Polyubiquitin Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.DB
Dot-blot analysis of all sorts of peptides using K11-linkage Specific Polyubiquitin antibody at 1:1000 dilution.| Product Name | K11-linkage Specific Polyubiquitin Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1:500-1:2000 DB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBB |
|---|---|
| Gene Synonyms | HEL-S-50 |
| Gene Full Name | ubiquitin B |
| Function | Polyubiquitination is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines with the C terminus of an adjoining ubiquitin are covalently attached to proteins. A crystal structure of K11-linked diubiquitin demonstrates a distinct conformation from K48- or K63-linked diubiquitin. It is important that K11-linked ubiquitin chains as critical regulators of mitotic protein degradation |
WB
Western blot analysis of various lysates using K11-linkage Specific Polyubiquitin Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
DB
Dot-blot analysis of all sorts of peptides using K11-linkage Specific Polyubiquitin antibody at 1:1000 dilution.| Application Notes | WB:1:500-1:2000 DB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
