WB
Expression of dFoxO in the fly brainlysates was detected with anti-dFoxO(1:3000-ditultion) combined with theECL Plus reagent.IP
Endogenous dFoxO from S2 cells wasimmunoprecipitated with anti-dFoxO.| Product Name | Anti Drosophila Forkhead Box Protein O (dFOXO) pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Forkhead transcription factor FoxO controls various cellular processes involved in cell cycle, cell death, metabolism and oxidative stress. Recent studies have also suggested FoxO is a key molecule for lifespan regulation of various organisms. Drosophila has a single FoxO gene (dFoxO), inactivation of which is dispensable for survival. However, dFoxO appears to regulate resistance to oxidative stress and aging in Drosophila. The activity of dFoxO is regulated by phosphorylation, ubiquitination and acetylation, which may cause multiple bands of dFoxO in western blotting using this antibody. This antibody detects endogenous levels of total dFoxO protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:3000 IP:1:10-1:100 |
|
| Species Reactivity | Drosophila |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Foxo |
|---|---|
| Gene Synonyms | 3143 Afx Akh anon-WO0118547.374 cg3143 CG3143 dfoxo dfoxO dFoxo dFoxO dFOXO Dfoxo DfoxO DFOXO Dmel\CG3143 DmQ95V5 fd88A FKHR foxO fOXO Foxo FoxO FOXO Foxo1 FOXO1 Q95V55 TORC |
| Gene Full Name | forkhead box, sub-group O |
| Gene Summary | Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including regulation of catabolic process; regulation of signal transduction; and regulation of transcription by RNA polymerase II. Acts upstream of with a negative effect on regulation of hemocyte proliferation and regulation of organ growth. Located in cytosol and nucleus. Is expressed in several structures, including embryonic segment; embryonic/larval midgut primordium; epidermis; fat body; and gut section. Used to study Parkinson's disease. Human ortholog(s) of this gene implicated in alveolar rhabdomyosarcoma. Orthologous to several human genes including FOXO4 (forkhead box O4). [provided by Alliance of Genome Resources, Apr 2025] |
| Molecular Weight(MW) | 87-100kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Expression of dFoxO in the fly brainlysates was detected with anti-dFoxO(1:3000-ditultion) combined with theECL Plus reagent.
IP
Endogenous dFoxO from S2 cells wasimmunoprecipitated with anti-dFoxO.| Application Notes | WB:1:1000-1:3000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
