IHC
Immunohistochemistry of paraffin-embedded human melanoma using TREM2 Antibody at dilution of 1:100.ICC/IF
Immunofluorescence staining of HepG2 cells with TREM2 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-TREM2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Triggering receptor expressed on myeloid cells 2 protein (19-174AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:20-1:200 ICC/IF:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TREM2 |
|---|---|
| Gene Synonyms | AD17 PLOSL2 TREM-2 Trem2a Trem2b Trem2c |
| Gene Full Name | triggering receptor expressed on myeloid cells 2 |
| Gene Summary | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
| Alternative Names | TREM 2 antibody TREM-2 antibody TREM2 antibody TREM2_HUMAN antibody TREM2a antibody TREM2b antibody TREM2c antibody Trggering receptor expressed on myeloid cells 2 antibody Trggering receptor expressed on myeloid cells 2a antibody Triggering receptor expressed on monocytes 2 antibody Triggering receptor expressed on myeloid cells 2 antibody |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein; Secreted. |
IHC
Immunohistochemistry of paraffin-embedded human melanoma using TREM2 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescence staining of HepG2 cells with TREM2 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:20-1:200 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
