WB
Western blot analysis of KMT1E / SETDB1 on different lysates with Rabbit anti-KMT1E / SETDB1 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: T-47D cell lysate, Lane 3: 293T cell lysate, Lane 4: HeLa cell lysate, Lane 5: K-562 cell lysate, Lane 6: COS-1 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: F9 cell lysate, Lane 9: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
KMT1E / SETDB1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-KMT1E / SETDB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-KMT1E / SETDB1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-KMT1E / SETDB1 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-KMT1E / SETDB1 antibody in Jurkat cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 30 seconds.| Product Name | KMT1E / SETDB1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SETDB1 aa 1-400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:200-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SETDB1 |
|---|---|
| Gene Synonyms | ESET KG1T KMT1E TDRD21 H3-K9-HMTase4 |
| Gene Full Name | SET domain bifurcated histone lysine methyltransferase 1 |
| Gene Summary | This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011] |
| Alternative Names | AU022152 antibody EC 2.1.1.43 antibody ERG-associated protein with SET domain antibody ESET antibody H3 K9 HMTase 4 antibody H3-K9-HMTase 4 antibody H3-K9-HMTase4 antibody Histone H3 K9 methyltransferase 4 antibody Histone H3-K9 methyltransferase 4 antibody Histone-lysine N-methyltransferase SETDB1 antibody AU022152 antibody EC 2.1.1.43 antibody ERG-associated protein with SET domain antibody ESET antibody H3 K9 HMTase 4 antibody H3-K9-HMTase 4 antibody H3-K9-HMTase4 antibody Histone H3 K9 methyltransferase 4 antibody Histone H3-K9 methyltransferase 4 antibody Histone-lysine N-methyltransferase SETDB1 antibody KG1T antibody KIAA0067 antibody KMT1E antibody Lysine N-methyltransferase 1E antibody MGC90670 antibody mKIAA0067 antibody SET domain bifurcated 1 antibody Set domain protein bifurcated 1 antibody SETB1_HUMAN antibody Setdb1 antibody |
| Molecular Weight(MW) | 143 kDa(Observed band size: 180 kDa) |
| Cellular Localization | Nucleus, Cytoplasm, Chromosome. |
WB
Western blot analysis of KMT1E / SETDB1 on different lysates with Rabbit anti-KMT1E / SETDB1 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: T-47D cell lysate, Lane 3: 293T cell lysate, Lane 4: HeLa cell lysate, Lane 5: K-562 cell lysate, Lane 6: COS-1 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: F9 cell lysate, Lane 9: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-KMT1E / SETDB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
KMT1E / SETDB1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-KMT1E / SETDB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-KMT1E / SETDB1 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-KMT1E / SETDB1 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-KMT1E / SETDB1 antibody in Jurkat cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 30 seconds.| Application Notes | WB:1:5000 IHC:1:200-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
