WB
Western blot analysis of Tartrate-resistant acid phosphatase type 5 on different lysates with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate, Lane 2: SK-MEL-28 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Tartrate-resistant acid phosphatase type 5 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Tartrate-resistant acid phosphatase type 5 aa 126-325 / 325. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACP5 |
|---|---|
| Gene Synonyms | HPAP TRAP TRAcP TRACP5a TRACP5b TrATPase |
| Gene Full Name | acid phosphatase 5, tartrate resistant |
| Gene Summary | This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] |
| Alternative Names | Type 5 acid phosphatase antibody Acid phosphatase 5 tartrate resistant antibody Acid phosphatase 5 tartrate resistant antibody ACP5 antibody EC 3.1.3.2 antibody MGC117378 antibody PPA5_HUMAN antibody serum band 5 tartrate-resistant acid phosphatase antibody SPENCDI antibody T5ap antibody Type 5 acid phosphatase antibody Acid phosphatase 5 tartrate resistant antibody Acid phosphatase 5 tartrate resistant antibody ACP5 antibody EC 3.1.3.2 antibody MGC117378 antibody PPA5_HUMAN antibody serum band 5 tartrate-resistant acid phosphatase antibody SPENCDI antibody T5ap antibody Tartrate resistant acid ATPase antibody Tartrate resistant acid phosphatase type 5 antibody Tartrate resistant acid phosphatase type 5 precursor antibody Tartrate-resistant acid ATPase antibody Tartrate-resistant acid phosphatase type 5 antibody TR AP antibody TR-AP antibody TRACP 5 antibody TRAP antibody TrATPase antibody Type 5 acid phosphatase antibody |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Lysosome. |
WB
Western blot analysis of Tartrate-resistant acid phosphatase type 5 on different lysates with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate, Lane 2: SK-MEL-28 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
