WB
Western blot analysis of FPR2 on different lysates with Rabbit anti-FPR2 antibody at 1/5,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Lane 3: J774A.1 cell lysate (20 µg/Lane), Lane 4: RAW264.7 cell lysate (20 µg/Lane), Lane 5: Mouse brain tissue lysate (40 µg/Lane), Lane 6: Mouse bone marrow tissue lysate (40 µg/Lane), Lane 7: Rat brain tissue lysate (40 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of THP-1 cells labeling FPR2 with Rabbit anti-FPR2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FPR2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of THP-1 cells labeling FPR2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | FPR2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human FPR2 aa 1-50. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:5000 ICC/IF:1:500 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FPR2 |
|---|---|
| Gene Synonyms | ALX ALXR HM63 FMLPX FPR2A FPRH1 FPRH2 FPRL1 LXA4R FMLP-R-II |
| Gene Full Name | formyl peptide receptor 2 |
| Gene Summary | Enables amyloid-beta binding activity; scavenger receptor binding activity; and signaling receptor activity. Involved in several processes, including cellular response to amyloid-beta; positive regulation of ERK1 and ERK2 cascade; and regulation of defense response. Located in cytoplasm and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ALXR antibody FMLP-R-I antibody FMLP-R-II antibody FMLP-related receptor I antibody FMLPX antibody Formyl peptide receptor 2 antibody Formyl peptide receptor related antibody Formyl peptide receptor-like 1 antibody FPR2 antibody FPR2_HUMAN antibody ALXR antibody FMLP-R-I antibody FMLP-R-II antibody FMLP-related receptor I antibody FMLPX antibody Formyl peptide receptor 2 antibody Formyl peptide receptor related antibody Formyl peptide receptor-like 1 antibody FPR2 antibody FPR2_HUMAN antibody FPR2A antibody FPRH1 antibody FPRH2 antibody FPRL1 antibody HM63 antibody Lipoxin A4 receptor antibody LXA4 receptor antibody LXA4R antibody N-formyl peptide receptor 2 antibody RFP antibody |
| Molecular Weight(MW) | 39 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of FPR2 on different lysates with Rabbit anti-FPR2 antibody at 1/5,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane), Lane 2: THP-1 cell lysate (20 µg/Lane), Lane 3: J774A.1 cell lysate (20 µg/Lane), Lane 4: RAW264.7 cell lysate (20 µg/Lane), Lane 5: Mouse brain tissue lysate (40 µg/Lane), Lane 6: Mouse bone marrow tissue lysate (40 µg/Lane), Lane 7: Rat brain tissue lysate (40 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of THP-1 cells labeling FPR2 with Rabbit anti-FPR2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FPR2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of THP-1 cells labeling FPR2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 ICC/IF:1:500 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
