WB
Western blot analysis of extracts from EC304 cells(heat-shock treatment), using GPR105 Antibody at 1/1000 dilution.
IHC
GPR105 Antibody at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.