WB
Western blot analysis of SIK1 using anti-SIK1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .ICC/IF
ICC/IF analysis of SIK1 using anti-SIK1 antibody and anti-Beta Tubulin antibody. SIK1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SIK1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody. The section was counterstained with DAPI (Blue).FCM
Flow Cytometry analysis of Jurkat cells using anti-SIK1 antibody). Overlay histogram showing Jurkat cells stained with Rabbit polyclonal antibody to Snf1lk/SIK1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIK1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Snf1lk/SIK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Snf1lk/SIK1 recombinant protein (Position: M1-Q783). Human Snf1lk/SIK1 shares 80.5% and 81.2% amino acid (aa) sequence identity with mouse and rat Snf1lk/SIK1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCMICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FCM:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIK1 |
|---|---|
| Gene Synonyms | MSK SIK DEE30 SIK-1 SIK1B SNF1LK |
| Gene Full Name | salt inducible kinase 1 |
| Gene Summary | This gene encodes a serine/threonine protein kinase that contains a ubiquitin-associated (UBA) domain. The encoded protein is a member of the adenosine monophosphate-activated kinase (AMPK) subfamily of kinases that play a role in conserved signal transduction pathways. A mutation in this gene is associated with early infantile epileptic encephalopathy 30. [provided by RefSeq, Nov 2016] |
| Alternative Names | Sik1 Salt-inducible kinase 1 salt inducible kinase 1 Myocardial SNF1-like kinase Msk |
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of SIK1 using anti-SIK1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .
ICC/IF
ICC/IF analysis of SIK1 using anti-SIK1 antibody and anti-Beta Tubulin antibody. SIK1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SIK1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody. The section was counterstained with DAPI (Blue).
FCM
Flow Cytometry analysis of Jurkat cells using anti-SIK1 antibody). Overlay histogram showing Jurkat cells stained with Rabbit polyclonal antibody to Snf1lk/SIK1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIK1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FCM:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
