WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ACACA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: NIH-3T3 Lane 3: HEK293IHC
Human breast carcinoma was stained with anti-ACACA rabbit antibodyICC/IF
Immunofluorescence analysis of HEK293. Picture A: ACACA antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+BWB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.| Product Name | ACACA Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACACA |
|---|---|
| Gene Synonyms | ACC ACAC ACC1 ACCA Acac1 hACC1 ACACAD ACCalpha ACACalpha |
| Gene Full Name | acetyl-CoA carboxylase alpha |
| Gene Summary | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ACACA ACAC ACC1 ACCA Acetyl-CoA carboxylase 1 ACC-alpha |
| Molecular Weight(MW) | 265 kDa |
| Cellular Localization | Cytoplasm, cytosol . |
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ACACA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: NIH-3T3 Lane 3: HEK293
IHC
Human breast carcinoma was stained with anti-ACACA rabbit antibody
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: ACACA antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:4000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
