WB
Western blot analysis of Thrombospondin 2 expression in A549 (A), Raw264.7 (B), PC12 (C) whole cell lysates.IHC
Immunohistochemical analysis of Thrombospondin 2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.ICC/IF
Immunofluorescent analysis of Thrombospondin 2 staining in 22RV1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).| Product Name | Anti-Thrombospondin 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Thrombospondin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | BovineChickenDogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | THBS2 |
|---|---|
| Gene Synonyms | TSP2 EDSCLL3 |
| Gene Full Name | thrombospondin 2 |
| Gene Summary | The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008] |
| Alternative Names | TSP2 Thrombospondin-2 |
| Molecular Weight(MW) | 129 kDa |
WB
Western blot analysis of Thrombospondin 2 expression in A549 (A), Raw264.7 (B), PC12 (C) whole cell lysates.
IHC
Immunohistochemical analysis of Thrombospondin 2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.
ICC/IF
Immunofluorescent analysis of Thrombospondin 2 staining in 22RV1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
