WB
Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: MDA-MB-231 cell lysate, Lane 3: Huh7 cell lysate, Lane 4: THP-1 cell lysate (negative), Lysates/proteins at 20 ug/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ENPP1 / PC1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ENPP1 aa 576-925. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ENPP1 |
|---|---|
| Gene Synonyms | M6S1 NPP1 NPPS PC-1 PCA1 ARHR2 COLED PDNP1 |
| Gene Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 1 |
| Gene Summary | This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008] |
| Alternative Names | Alkaline phosphodiesterase 1 antibody ARHR2 antibody COLED antibody E-NPP 1 antibody Ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 antibody ENPP1 antibody ENPP1_HUMAN antibody Ly 41 antigen antibody M6S1 antibody Alkaline phosphodiesterase 1 antibody ARHR2 antibody COLED antibody E-NPP 1 antibody Ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 antibody ENPP1 antibody ENPP1_HUMAN antibody Ly 41 antigen antibody M6S1 antibody Membrane component chromosome 6 surface marker 1 antibody NPP1 antibody NPPase antibody NPPS antibody Nucleotide pyrophosphatase antibody PC 1 antibody PC-1 antibody PCA1 antibody PDNP1 antibody Phosphodiesterase I/nucleotide pyrophosphatase 1 antibody Plasma cell membrane glycoprotein 1 antibody Plasma-cell membrane glycoprotein PC-1 antibody |
| Molecular Weight(MW) | 105 kDa(Observed band size: 140 kDa) |
| Cellular Localization | Cell membrane, Basolateral cell membrane, Secreted. |
WB
Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: MDA-MB-231 cell lysate, Lane 3: Huh7 cell lysate, Lane 4: THP-1 cell lysate (negative), Lysates/proteins at 20 ug/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
