IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Pericentrin antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).ICC/IF
HeLa cells using Pericentrin antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using Pericentrin antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | Pericentrin Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|
| Species Reactivity | CanineHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCNT |
|---|---|
| Gene Synonyms | KEN PCN MOPD2 PCNT2 PCNTB PCTN2 SCKL4 |
| Gene Full Name | pericentrin |
| Gene Summary | The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
| Alternative Names | PCNT Pericentrin-B Pericentrin B |
| Molecular Weight(MW) | 378 kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Microtubule |
IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Pericentrin antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
ICC/IF
HeLa cells using Pericentrin antibody at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using Pericentrin antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
