IHC
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CACNA2D1 Antibody at dilution of 1:100.ICC/IF
Immunofluorescence staining of Hela cells with CACNA2D1 Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-CACNA2D1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Voltage-dependent calcium channel subunit alpha-2/delta-1 protein (577-717AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:20-1:200 ICC/IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CACNA2D1 |
|---|---|
| Gene Synonyms | CACNA2 CCHL2A DEE110 CACNL2A LINC01112 lncRNA-N3 |
| Gene Full Name | calcium voltage-gated channel auxiliary subunit alpha2delta 1 |
| Gene Summary | The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014] |
| Alternative Names | CA2D1_HUMAN antibody CACN A2 antibody CACNA2 antibody Cacna2d1 antibody CACNL2A antibody Calcium channel L type alpha 2 polypeptide antibody Calcium channel voltage dependent alpha 2/delta subunit 1 antibody CCHL2A antibody Dihydropyridine receptor alpha 2 subunit antibody Dihydropyridine sensitive L type calcium channel alpha 2/delta subunit antibody Dihydropyridine sensitive L type calcium channel subunits alpha 2/delta antibody L type calcium channel subunit alpha 2 antibody MHS 3 antibody MHS3 antibody Voltage dependent calcium channel subunit alpha 2/delta 1 antibody Voltage gated calcium channel subunit alpha 2/delta 1 antibody Voltage-dependent calcium channel subunit delta-1 antibody Voltage-gated calcium channel subunit alpha-2/delta-1 antibody |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
IHC
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CACNA2D1 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescence staining of Hela cells with CACNA2D1 Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:20-1:200 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
