WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD10 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.IHC
Human kidney was stained with anti-CD10 rabbit antibody.IHC
Mouse kidney was stained with anti-CD10 rabbit antibody.IHC2
Rat kidney was stained with anti-CD10 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: CD10 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | CD10 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1000-1:4000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MME |
|---|---|
| Gene Synonyms | NEP SFE CD10 CALLA CMT2T SCA43 |
| Gene Full Name | membrane metalloendopeptidase |
| Gene Summary | The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017] |
| Alternative Names | MME EPN Neprilysin Atriopeptidase Common acute lymphocytic leukemia antigen CALLA Enkephalinase Neutral endopeptidase 24.11 NEP Neutral endopeptidase Skin fibroblast elastase SFE CD10 |
| Molecular Weight(MW) | 86 kDa(Observed band size: 100 kDa) |
| Cellular Localization | Membrane |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD10 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-CD10 rabbit antibody.
IHC
Mouse kidney was stained with anti-CD10 rabbit antibody.
IHC2
Rat kidney was stained with anti-CD10 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: CD10 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1000-1:4000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
