WB
A549 cells were subjected to SDS PAGE followed by western blot with Stomatin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
HeLa cells were subjected to SDS PAGE followed by western blot with Stomatin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
human liver tissue were subjected to SDS PAGE followed by western blot with STOM antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
human lung tissue were subjected to SDS PAGE followed by western blot with STOM antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Stomatin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Stomatin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-Stomatin (IP: 3ug; Detection: 1:300) with HeLa cells lysate 2800ug.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Stomatin antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | Stomatin Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human STOM protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STOM |
|---|---|
| Gene Synonyms | BND7 EPB7 EPB72 |
| Gene Full Name | stomatin |
| Gene Summary | This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] |
| Alternative Names | STOM BND7 EPB7 EPB72 Erythrocyte membrane protein band 7.2 |
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Membrane |
WB
A549 cells were subjected to SDS PAGE followed by western blot with Stomatin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
HeLa cells were subjected to SDS PAGE followed by western blot with Stomatin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
human liver tissue were subjected to SDS PAGE followed by western blot with STOM antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
human lung tissue were subjected to SDS PAGE followed by western blot with STOM antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Stomatin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Stomatin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-Stomatin (IP: 3ug; Detection: 1:300) with HeLa cells lysate 2800ug.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Stomatin antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
