WB
Sample: Primary: Anti-NPY1R (bs-1070R) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution,IF-P
Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Product Name | NPY1R Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human NPY1R : 221-270/384 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-2000 IF-P:1:100-500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NPY1R |
|---|---|
| Gene Synonyms | NPYR NPY1-R |
| Gene Full Name | neuropeptide Y receptor Y1 |
| Gene Summary | This gene belongs to the G-protein-coupled receptor superfamily. The encoded transmembrane protein mediates the function of neuropeptide Y (NPY), a neurotransmitter, and peptide YY (PYY), a gastrointestinal hormone. The encoded receptor undergoes fast agonist-induced internalization through clathrin-coated pits and is subsequently recycled back to the cell membrane. Activation of Y1 receptors may result in mobilization of intracellular calcium and inhibition of adenylate cyclase activity. [provided by RefSeq, Aug 2013] |
| Alternative Names | NPY1-R NPYR Y1-R NPY-1 NPY1R_HUMAN NPY1R NPYY1 NPY1R_MOUSE NPY1R_RAT FC5 neuropeptide Y receptor Y1 |
| Molecular Weight(MW) | 44 kDa(Observed band size: 60 kDa) |
| Cellular Localization | Cell membrane,Membrane |
WB
Sample: Primary: Anti-NPY1R (bs-1070R) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution,
IF-P
Tissue/cell: rat myocardium tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-NPY1R Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Application Notes | WB:1:500-2000 IF-P:1:100-500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
