WB
Western blot analysis of OTULIN using anti-OTULIN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OTULIN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OTULIN at approximately 40-45 kDa. The expected band size for OTULIN is at 40 kDa.IHC
IHC analysis of OTULIN using anti-OTULIN antibody. OTULIN was detected in a paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of OTULIN using anti-OTULIN antibody. OTULIN was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of OTULIN using anti-OTULIN antibody . OTULIN was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of 293T cells using anti-OTULIN antibody . Overlay histogram showing 293T cells stained with rabbit anti-OTULIN Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTULIN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to OTULIN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human OTULIN recombinant protein (Position: A49-L352). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 FCM:1:50-1:200 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTULIN |
|---|---|
| Gene Synonyms | GUM AIPDS AIPDSA IMD107 FAM105B |
| Gene Full Name | OTU deubiquitinase with linear linkage specificity |
| Gene Summary | This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients. [provided by RefSeq, Sep 2016] |
| Alternative Names | OTU deubiquitinase with linear linkage specificity AIPDS FAM105B GUM OTULIN |
| Molecular Weight(MW) | 40 kDa(Observed band size: 40-45 kDa) |
| Cellular Localization | Cytoplasm |
WB
Western blot analysis of OTULIN using anti-OTULIN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OTULIN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OTULIN at approximately 40-45 kDa. The expected band size for OTULIN is at 40 kDa.
IHC
IHC analysis of OTULIN using anti-OTULIN antibody. OTULIN was detected in a paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of OTULIN using anti-OTULIN antibody. OTULIN was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of OTULIN using anti-OTULIN antibody . OTULIN was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-OTULIN Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of 293T cells using anti-OTULIN antibody . Overlay histogram showing 293T cells stained with rabbit anti-OTULIN Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTULIN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 ICC/IF:1:50-1:400 FCM:1:50-1:200 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
