WB
Various lysates were subjected to SDS PAGE followed by western blot with S100A14 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
WB result of S100A14 antibody (1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells.IHC
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using S100A14 antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using S100A14 antibody at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A14 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-S100A14 (IP: 4ug; Detection: 1:1000) with MCF-7 cells lysate 760 ug.| Product Name | S100A14 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human S100A14 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:500-1:2000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S100A14 |
|---|---|
| Gene Synonyms | BCMP84 S100A15 |
| Gene Full Name | S100 calcium binding protein A14 |
| Gene Summary | This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011] |
| Alternative Names | S114 S100A15 S100 calcium-binding protein A14 Protein S100-A14 Protein S100 A14 |
| Molecular Weight(MW) | 12 kDa(Observed band size: 10-12 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with S100A14 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
WB result of S100A14 antibody (1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells.
IHC
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using S100A14 antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using S100A14 antibody at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A14 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-S100A14 (IP: 4ug; Detection: 1:1000) with MCF-7 cells lysate 760 ug.| Application Notes | WB:1:500-1:2000 IHC:1:500-1:2000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
