WB
Western blot analysis of Cubilin using COLO205 whole cell lysates.ICC/IF
Cubilin Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to Cubilin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Cubilin, corresponding to a region within N-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:3000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUBN |
|---|---|
| Gene Synonyms | IGS IFCR IGS1 MGA1 gp280 |
| Gene Full Name | cubilin |
| Gene Summary | Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008] |
| Alternative Names | 460 kDa receptor cubilin (intrinsic factor-cobalamin receptor) Cubilin Cubilin precursor Cubn CUBN_HUMAN IFCR Intestinal intrinsic factor receptor intrinsic factor B12-receptor Intrinsic factor-cobalamin receptor Intrinsic factor-vitamin B12 receptor megaloblastic anemia 1 MGA1 |
| Molecular Weight(MW) | 399 kDa |
| Cellular Localization | Apical cell membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein. Membrane>Coated pit. Endosome. Lysosome membrane>Peripheral membrane protein. |
WB
Western blot analysis of Cubilin using COLO205 whole cell lysates.
ICC/IF
Cubilin Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:1000-1:3000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
