WB
Various lysates were subjected to SDS PAGE followed by western blot with Cryptochrome 1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Cryptochrome 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Cryptochrome 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CRY1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRY1 |
|---|---|
| Gene Synonyms | DSPD PHLL1 |
| Gene Full Name | cryptochrome circadian regulator 1 |
| Gene Summary | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014] |
| Alternative Names | CRY1 CRY 1 Cryptochrome-1 |
| Molecular Weight(MW) | 66 kDa(Observed band size: 60-66 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Cryptochrome 1 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Cryptochrome 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
